Ferris Boyle was the CEO of GothCorp, and was reputed to be a humanitarian. In reality, however, Boyle was a stone-cold, unethical sociopath, whose 'legitimate projects' were actually a front to cover up his illegal and dangerious weapons manufacturing operations, which came to fruition through a combination of extortion, embezzlement, abduction, and even murder.
Ferris Boyle secretly conducted research into highly illegal and dangerous cryogenic weaponry, and kept most of his staff out of the loop. Eventually, he learned that one of GothCorp's scientists, Dr. Victor Fries, had successfully managed to place his dying wife, Nora Fries, into cryostasis, was intrigued by that, and offered him a deal: Fries would work on his cryogenic weapons program, and in exchange, Ferris would supply him with funding to finding a cure for the terminal illness Huntington's Chorea, which Nora was afflicted with.
Fries, desperate to save his wife, agreed to the deal. However, Boyle had absolutely no intention of actually fulfilling his end of the bargain and was far more interested in using Nora as a test subject in order to discover and research additional cryogenic techniques to apply to his projects and weaponize her condition. Fries, upon discovering that, decided to take matters into his own hands, and secretly conducted his research on the cure. Boyle eventually discovered that, had Fries brought to the lab, confiscated Nora's capsule, and had him beaten. Boyle also accidently fired a prototype Cryogenic Cannon, which caused an explosion that trapped a security guard and Fries. Boyle left the lab staff for dead, disposed of his gun, had the place quarantined, believed that all of the witnesses were dead, and covered up the event.
Some time later, on New Years Eve, Boyle was presented with the Humanitarian of the Year Award by the Wayne Foundation, with Bruce Wayne even believing that he would be a good hope for Gotham City. However, the ceremony was interrupted with the arrival of Mr. Freeze, alongside The Penguin's Henchmen, who intended to abduct Boyle so that they could use his security clearance to access Nora's capsule and get her out of GothCorp. Upon Boyle's capture, he was then dragged over to the Propellant Research Wing at GothCorp and was then taken hostage when Batman tried to rescue him. Freeze then told Boyle to supply him with the security codes for Nora. When Boyle refused, Freeze threatened to freeze him so that he could experience Nora's fate.
Batman eventually caught up with Freeze, and informed him that he knew the truth about what Boyle did to him (having investigated the quarantined lab earlier while searching for components for a cryodrill to access the lab). Boyle tried to falsely state that it was an accident, with Freeze going berserk and throwing him into a cryogenic capsule. After Freeze was defeated by Batman, Boyle revealed his true nature and proceeded to "thank" Batman for doing the heavy lifting and aimed a cryogenic Jetstream at Freeze, with Batman getting in the way of it. Boyle then revealed his intention to leave Batman for dead to silence him of his discovery.
Boyle then sadistically beat a pleading Freeze with a piece of pipe that he tore from his suit and taunted him that he's going to let him live long enough to watch Nora die. Batman managed to free himself due to the XE Suit and beat Boyle into unconsciousness, and also told him to "take a seat" in disgust for the "humanitarian." Because of evidence supplied by Batman regarding Boyle's crime, he was arrested alongside Freeze and Penguin.
Boyle was referenced via the Riddle: "Who can Boyle and Freeze at the same time" and Mr. Freeze's Interview Tapes. Freeze's antagonism with The Joker in the same game (although for slightly different reasons) also mirrored that of Boyle (who, coincidentally, were both voiced by Mark Hamill).