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Gambol's Thugs were a syndicate of criminals, in part a combination of illegal gamblers, racketeers and other African-American gangsters, who served under Gambol.
One of Gambol's men was murdered by the Joker, who slammed his face into a pencil, which stabbed through his eye, eraser first.
The Joker was brought to Gambol by some bounty hunters, killed two of Gambol's men, murdered Gambol, and ordered his own thugs to watch over Gambol's remaining men until they had fought each other to the death with half of a pool stick.